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Paula Martínez-Pérez     Other 
2
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36
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12
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Distribution of Articles published per year 
(2018)
Conference papers
CONFERENCE-ARTICLE 22 Reads 0 Citations <strong>Commercial Track-Etched Polycarbonate Membrane as Optical Chemical Sensors</strong> Paula Martínez-Pérez, Jaime García-Rupérez Published: 14 November 2018
doi: 10.3390/ecsa-5-05716
DOI See at publisher website ABS Show/hide abstract

Track-etched polycarbonate membranes (TEPM) are commercially available membranes typically used in particle filtration due to the pores present in their surfaces. This porous structure reminds of that of a Fabry-Pérot interferometer made on porous silicon, an optical structure long employed in optical chemical sensing. Because of this morphological similarity, we hypothesized that TEPM could exhibit a similar optical response and thus being useful for creating simpler to fabricate, easily available and low cost chemical sensors. To asses this hypothesis, we investigated the optical response of TEPM in the infrared range, improved it by chemically attaching the membranes to a silicon flat surface and then, performed reflectivity measurements in presence of different concentrations of ethanol.

When exposing a TEPM to a change of the refractive index of the medium it is surrounded by (air) by placing a drop of ethanol, with a higher refractive index, we can observe a shift of its spectrum towards higher wavelengths. This indicates the presence of the solvent, and we could check that the bigger the concentration, the bigger the magnitude of that shift. Furthermore, when the solvent is evaporated the spectrum returns to its initial position, which allowed us to perform different concentration sensing steps using the same sample. These promising results, although early, could indicate the utility of these membranes to easily fabricate cheap chemical sensors and, probably, optical biosensors as their surface can be chemically modified.

CONFERENCE-ARTICLE 14 Reads 0 Citations <strong>Affinity and selectivity of cardiac versus skeletal troponin I towards cardiac troponin I antibody: a computatio... Jad SABEK, Paula Perez, Jaime Ruperez Published: 14 November 2018
doi: 10.3390/ecsa-5-05719
DOI See at publisher website ABS Show/hide abstract

Cardiac troponin I (cTnI) is currently the gold-standard biomarker for the fast and early detection of a myocardial failure. Within this context, in this work we report a computational study of the interactions of the cTnI antibody (αcTnI) capture probe with cTnI and its principal interferon, skeletal troponin I (sTnI). This study allows having a better understanding of those biochemical interactions and to computationally predict the binding performance and the selectivity of the αcTnI to cTnI versus sTnI. This information is very relevant for the development of analysis systems for myocardial failure diagnosis based on cTnI detection.

The computational study was performed using different simulation platforms. FTSite and FTMap were used for the determination and mapping of the binding sites sequences [1]. Then, FTDock and pyDock were used to study the molecular dockings [2]. Thus, several energies parameters were generated and represented as well.

This study can also be applied to a wide range of different scenarios were other targets (e.g., lipids, oligonucleotides, etc.) or other applications (e.g., pharmacological drug design) are considered.

References:

[1] Y. Yuan, J. Pei, L. Lai. Curr. Pharm. Des. 2016, 19 (12), 2326-2333.

[2] M. Cheng, L. Blundell, J. Fernandez-Recio. Proteins. 2007, 68, 503-515.

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